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How many bases can we expect for large insert clone sequencing?

April 26, 2017bases clone expect insert large sequencing

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How many bases can we expect for large insert clone sequencing?

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Generally, if the template is very clean, we can read 500 (+/-50) basepairs for one single-stranded sequencing run on an average template. “Non-average” templates include high G/C, high A/T, microsatellite and those with a long mononucleotide stretch.