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How many basepairs can I expect from one sequencing reaction of a PCR product?

April 26, 2017basepairs expect PCR Product REACTION sequencing

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How many basepairs can I expect from one sequencing reaction of a PCR product?

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This depends on the concentration and quality of the initial PCR. In most instances, we can sequence 700 bp very cleanly. We have also had some templates where we have read over 800 bp very accurately.