How long should I wait after transfection before enriching my transfected cells, validating knock down, and looking for phenotypic changes?
We recommend starting your FACS-based enrichment or your neomycin (or puromycin) selection 24 to 48 hours after transfection with the SureSilencing™ shRNA plasmids. This amount of time is typically sufficient to begin detecting expression of the selectable markers (GFP, puromycin-resistance, neomycin-resistance), and observe the onset of the shRNA-mediated knock down of gene expression.
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- How long should I wait after transfection before enriching my transfected cells, validating knock down, and looking for phenotypic changes?
