How long is the initial denaturation time for CleanAmp(TM) Primers?
A – The initial denaturation time is 2 to 10 minutes at 94C for standard thermal cycling protocols. If used in combination with a chemically modified DNA polymerase, such as AmpliTaq Gold DNA polymerase, an initial denaturation of 10 to 20 minutes may be required. For fast cycling, shorter denaturation times at 94C can be employed.
