How long is chromatin shearing?
The time of shearing depend on many factors, like: cell type, cell density, volume of the sample in the tube and amount of the tubes during the sonication, fixation time, concentration of detergent in the shearing buffer, etc. So it is important to optimise the sonication conditions by each Bioruptor® user. Generally the shear time is 15 minutes. It is possible to shear for as little as 5 minutes and up to 30 minutes maximum (for longer fixation period: a longer shearing is needed).
