How long does jetPEI™-FluoF or jetPEI™-FluoR fluoresce in cells?
The fluorochromes (F or R) conjugated to jetPEI™ are always fluorescent. During the process of transfection, the fluorescence will be visible as long as jetPEI™ is present in the cells. However, note that the fluorescence is degraded by the laser of the microscope and by natural light. Thus leaving transfected cells exposed to the microscope light for too long drastically decreases fluorescence intensity. As a result, the reagents should be stored in the dark. In experiment using jetPEI™-Fluos to transfect plasmid DNA, we observed fluorescence up to 72 hours after transfection.
