How long can we store membranes in 20% ethanol after hydration?
We recommend storing them no longer than 4 weeks in 20% ethanol at 4ºC if the initial buffer was sterile. The ethanol is initially added because it helps to remove any glycerin which is added to the membrane during manufacturing to help promote hydration. The key point is that one must avoid any bacterial growth as they may produce cellulases that modify/destroy the membranes.
