How long can I store the CT Conversion Reagent in the MethylCode™ Bisulfite Conversion Kit after it has been dissolved?
The CT Conversion Reagent can be stored for one day at room temperature, one week at 4 C, one month at -20 C, and for up to 6 months at -80 C without a noticeable loss in quality. The protocol describes dissolving the CT Conversion Reagent in a total of 1.25 mL, and it indicates that it should be sufficient for 10 reactions. However, the protocol later mentions that 130 μ L is required for each reaction, which adds up to 1.3 mL. Is this a mistake in the protocol? The powdered chemicals will add to the total volume of the final solution. The volume should be greater than 1.4 mL after completely resuspending the CT Conversion Reagent as indicated in the protocol.
