How is time divided to calculate the kinetics functions?
The minimum is 1 bin/second and the maximum is a total of 512 bins. However, if you are computing certain statistics, FlowJo tries to adjust bin size to accommodate the stats. For example, if you are computing a percentile, it wants to have at least 10 events in each bin on the smaller side of the percentile. (For example, if you are computing median, FlowJo tries to have at least 20 events per bin; if you are doing the 10th or 90th percentile, it tries to have at least 100 events per bin, on average). For mean calculations, FlowJo wants to have at least 10 events per bin. If you are doing statistics on the events above threshold, these event numbers are increased by a factor of 3. Once FlowJo knows the minimum average number of events per bin necessary for the statistics, it computes the width of the bin accordingly. For example, if it wants 100 events per bin, and there are 10,000 events, then it will try to make 100 bins, as long as the absolute criteria (1 bin/second, max of 512 bi
