How is the standard result, incubated at 37C, determined?
The quantification of the bacterial tests initially developed was based on the time taken for a large range of 100 mL water or milk samples to turn fully from blue to pink for coliforms, and then later, to white for E. coli. The time taken when the sample was incubated at 37C was compared to plate counts using Chromocult Coliform Agar (CCA – Merck, Darmstadt, Germany) either as standard plates or after membrane filtration. A good description of the CCA test is given in Gonzalez, Tomagnini, Olmos and de Sousa (2003). This also provides a matrix of the coliform and non-coliform responses to the media compared to VRBA plate counts. The advantage of the CCA test procedure is that both coliforms and E. coli can be specifically determined in the same test at the same time. A physical example of the CCA test can be compared with Coliquik test results as shown below. A further paper of relevance to the difference between CCA and standard tests, fluorescence tests and CCA variants is specifical
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- How is the standard result, incubated at 37C, determined?
