How is the SEM different from a light microscope?
Unlike a light microscope, the SEM uses electrons instead of white light to view the specimen. With the SEM you can only view inanimate materials, but you can magnify over 100,000 times. Rather than seeing “through and inside” a living organism, as you would with a light microscope, you are viewing the surface details. SEM images are in black and white because only light carries colour information, but the images can have false colour added to them using computer software. 2. How does it work? The scanning electron microscope (SEM) provides a three-dimensional high-resolution image of cells and tissues. In SEM the surface of the tissue is studied. Tissues prepared for SEM are usually chemically fixed using Glutaraldehyde and then dehydrated in ethanol before being dried. Drying at critical point has become the preferred method. The tissue is introduced into liquid carbon dioxide which is then brought to its critical point, which is the combination of pressure and temperature at which t
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