How is the sample processed and stored?
Cord blood is processed using a red blood cell depletion method to remove most of the red blood cells. DMSO, a cryoprotectant, is added to the cord blood prior to freezing. Freezing is accomplished using a “controlled-rate freezer.” This freezer gradually freezes the cord blood over a period of approximately one hour. It also provides a record of the entire freezing process. The cord blood is then stored in a dual compartment cryocyte bag in liquid nitrogen. Each cryocyte bag is placed in an additional, slightly larger bag that is sealed using a special heat sealer. This overwrap provides added protection to your cord blood by providing another barrier to cross-contaminants. It will also help contain the cord blood in the unlikely case that any breakage occurs. The bag is held in the vapor phase of liquid nitrogen during the quarantine phase. Once all required tests are completed and results found acceptable, the cord blood is transferred to a liquid nitrogen storage tank.
