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How is the poly(A) tail added during reverse transcription using the miScript PCR System?

April 26, 2017added miscript PCR Poly reverse system tail transcription

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How is the poly(A) tail added during reverse transcription using the miScript PCR System?

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During the reverse transcription step, a poly(A) tail is added at the 3′ end of the miRNA by poly(A) polymerase using ATP present in the miScript Reverse Transcription Mix (see figure “miScript principle”).