How is the database of alternative spliceforms constructed?
A batch process retrieves all of the complete coding mRNA records from RefSeq and GenBank. NCBI Gene data files are used to associate transcript records to genes. The transcripts are aligned to chromosomal sequence to determine the exon structure of the transcript. Quality assurance is done to eliminate poor quality transcript sequences and transcripts with duplicate exon structure are eliminated. The chromosomal coordinates of each exon of the remaining transcripts are stored in a relational database. A detailed description of the build process can be found in this document: SpliceCenter_DataBuild.
