How is target-specific selection achieved?
Sequence selection can be achieved by hybridization using complementary capture probes or by sequence-specific ligand molecules such as DNA/RNA binding molecules including small ligands, drug molecules, proteins/antibodies/peptides, aptamers, etc which bind to the sequence of interest. On-Chip or Microarray-Based Target Selection Capture probes are immobilized on surface for hybridization. Samples are hybridized to a microarray containing capture probes designed to target specific regions of interest in any genome (or transcriptome). Undesired sequences are washed off and captured target sequences are recovered by eluting from the chip. These selected target sequences are ready for high-throughput sequencing or they can be further processed. In-Solution Target Selection Capture probes can be used for solution hybridization. One method involves using oligonucleotides (OligoMix) in solution as capture probes which are designed to target specific genomic/sequence regions of interest. Afte
