How is SYBR Green JumpStart Taq ReadyMix different from ABI’s SYBR Green PCR Master Mix?
The formulation is very similar. ABI’s product contains AmpliTaq Gold® (chemically inactivated hot-start polymerase), SYBR Green dye, deoxynucleotides and optimized buffer. A ROX reference dye solution is also included. Sigma’s product is designed to perform comparable to the ABI product, using JumpStart Taq for antibody-inactivated hot start PCR. A proprietary reference dye solution is provided with Sigma’s SYBR Green JumpStart Taq ReadyMix. The solution may be used as a reference for baseline fluorescence, using the same instrument settings as for ROX.
Related Questions
- Why is the QuantiFast denaturation step different for PCR and RT-PCR runs in the two-step protocol for the ABI 7500 and other cyclers?
- Is the master mix of QuantiFast Kits for real-time PCR aliquoted into several tubes to prevent cross-contamination?
- How is SYBR Green JumpStart Taq ReadyMix different from ABI’s SYBR Green PCR Master Mix?
