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How is specificity achieved for mature miRNA over pre-miRNA?

April 26, 2017achieved Mature mirna pre-mirna Specificity

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How is specificity achieved for mature miRNA over pre-miRNA?

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10 Posted

Specificity is achieved in the Extension step (in the Add MEL step) and cycling. The Extension step favors mature miRNAs because longer sequences will not achieve complete extension to the PCR primer portion of the Make CSP poly-dT primer to the same degree as mature miRNAs.