How is resequencing done?
We amplify the specified gene(s) or region(s) requested by the investigator using PCR. Four types of coverage can be requested: a) Protein coding exons only, b) All exons (UTR and coding) only, c) NHLBI Standard coverage which consists of exons, conserved non-coding sequences plus 2 kb upstream and 2 kb downstream of the gene(s) across a region(s), d) NHLBI Full (complete) coverage of the gene(s) or across the region(s) including all coding and non-coding sequences, plus 2 kb upstream and 2 kb downstream of the gene(s) or region(s). In addition, only regions refractory to PCR amplification or sequencing (e.g., low-complexity repeats) are excluded. For these four options precomputed estimates for the sequences have been identified and calculated to aid the investigator in defining the size of their overall project.
Related Questions
- WHAT IS THE TYPICAL ERROR RATE ASSOCIATED WITH USAGE OF HYB & SEQ RESEQUENCING? HOW DO REGIONS OF SECONDARY STRUCTURE AFFECT HYB & SEQ PERFORMANCE?
- CAN I USE HYB & SEQ TO PERFORM RESEQUENCING IN BOTH FORWARD AND REVERSE DIRECTIONS?
- Are there limits on the size of the study I can propose for resequencing?
