How is one transfection reaction defined for the GenePORTER reagents?
One transfection reaction for the GenePORTER reagents is defined as delivery of 2 µg of DNA in one well of a 6-well plate or in a 35mm dish of cells. Please note that GenePORTER reagent transfection conditions are not based on the surface area or the volume of the culture dish, but rather the number of cells plated. Please refer to the protocols accompanying the product.