How is it possible to obtain accurate results without filtration?
Direct, in situ UV measurements with the OPT-DISS system are only potentially affected by solution turbidity — not particulates. If, with your manual, off-line UV method, you were only filtering to remove particulates that would release active into your measurement cuvettes, then the in situ measurements would accurately reflect the concentration of active in the vessel without having to correct for turbidity effects. In fact you would be avoiding the possible detrimental effects of filtration such as removing leachable materials from the filter and/or adsorbing active drug ingredient onto the filter.
