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How is it possible to avoid the loss of OncoQuick®-enriched tumor cells during a following cytospin step?

April 26, 2017avoid cells cytospin loss possible step tumor

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How is it possible to avoid the loss of OncoQuick®-enriched tumor cells during a following cytospin step?

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• use a careful cytospin protocol • adapt speed of cytofuge to sensitive cells (600 to 800 rpm with Shandon-Cytofuge) to avoid disruption of cells • use concentrated cell suspensions (1 000 000 cells/mL; 100 – 200 µl cell suspension/slide) • lengthen centrifugation time (6-10 min)