How is gene/isoform expression level calculated?
By applying splicing graph, we track every transcript supporting each AS event. Integrating tissue information of EST libraries (from G enBank), for each isoform, the distribution of supporting transcripts in different tissues, developmental stages and health status were tabulized and color-enhanced. We also provide the total number of transcripts of the gene as a control, so that the user can see if an isoform is enriched in a particular condition and if this enrichment is simply due to more EST libraries in the tissue. Several things should be noted: First, mRNA sequences and RefSeq transcripts do not have tissue information, so they were listed separately on the left (not color-coded). Second, sometimes, the number of supporting transcripts in the table is less than the total number given in the summary section. This is becuase some EST libraries do not have tissue information and the sequences in these libraries are not counted in the tabular results.
