How is flow cell clustering conducted on MiSeq?
Cluster generation follows the same process that occurs on the cBot in preparation for runs on the HiSeq or Genome Analyzer, except on MiSeq all reagents for cluster generation, sequencing, and paired-end chemistry are loaded onto the sequencer prior to the start of the run. After the run is started, the system proceeds to cluster generation followed by sequencing and paired end chemistry (if selected).