How is Dicer different from using RNase III to cleave the dsRNA?
The RNase III enzyme apparently cleaves long dsRNA into smaller (12-15 nucleotide) fragments than Dicer. There are some reports that these resulting fragments may be effective at generating an RNAi effect, although it appears that much more product needs to be transfected to achieve this knockdown. The Dicer enzyme is a natural component of the endogenous RNAi pathway in eukaryotes, and purified recombinant Dicer used in an optimal protocol cleaves the long dsRNA to the specific size known to be effective for generating the knockdown response.
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