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How is a Phenotype MicroArray™ experiment executed?

April 26, 2017executed experiment phenotype

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How is a Phenotype MicroArray™ experiment executed?

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Bacteria are grown on Biolog BUG plus blood agar plates. Single colonies are swabbed and suspended in inoculating fluid. Cell suspensions are pipetted at 100 ul per well into half area/96 well Biolog PM panels. Panels are incubated in the OmniLog® incubator reader and data is collected, organized and stored over a timecourse.