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How important is the OD600 for making competent cells?

April 26, 2017cells Competent od600

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How important is the OD600 for making competent cells?

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You need to harvest the bacteria when they are in early-mid log phase, so the OD is important. We use an OD600 of 0.55-0.6. Please be aware that different spectrophotometers vary considerably, so you have to determine the optimal OD for your own spec.