How important is the buffer used to dissolve the DNA?
The buffer used to dissolve DNA is important. The pH of the DNA preparation should be around 8.2 in order to prevent depurination during the initial heat treatment at 98°C. Some salts or other impurities in the sample might inhibit the ligase or polymerase enzyme. We recommend the use of DNA solutions in TE (10 mM Tris-HCl pH 8.2 + 0.1-1 mM EDTA). Do not use more than 1 mM EDTA in the sample solution!
