How important is normalization in miRNA detection experiments?
For accurate and reproducible results in miRNA detection by real-time PCR, it is necessary to normalize the amount of target miRNA by using a suitable endogenous reference RNA. This relative quantification approach is an important control which corrects for factors that could otherwise lead to inaccurate quantification. These factors include variation in quantity of input RNA, possible RNA degradation or presence of inhibitors in the RNA samples, and differences in sample handling. Normalization also allows results from different experiments and samples to be compared directly. miScript PCR Controls are used for normalization with the miScript PCR System.
