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How important is it to rinse the slides extensively with DI water after the slides are subject to blocking buffer and/or wash buffer?

April 26, 2017blocking buffer di extensively rinse slides Subject wash water

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How important is it to rinse the slides extensively with DI water after the slides are subject to blocking buffer and/or wash buffer?

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It is extremely important to rinse the slide extensively with DI water. Any residual reagents on the slide surface may cause non-uniform background. Rinsing the slides with DI water extensively helps achieve better background uniformity.