How i can prepare 100mM TE buffer(10 mM Tris-HCL PH 8,1mM EDTA?
You can buy TE buffer from a supplier. If you need to make it… combine Tris-HCL and EDTA at the right concentrations, then titrate it to pH 8.1 with NaOH. The chemicals you have will say what concentration (liquid) or molecular weight (solid) they are at. To give you exact numbers I’d need to know what you’re starting with and how much volume you need. For example, to make 1L of TE from Tris-HCL crystals with MW 158 and EDTA at 10mM: a bit under 900 mL water 1.58 g Tris-HCL 100 mL EDTA add NaOH until the pH reaches 8.1 add water until 1 L total. I am confused by your asking for “100mM”. TE buffer should have the components at the individual concentrations you listed.
