How efficient is jetPEI™ for the transfection of Jurkat cells?
jetPEI™ is among the most efficient chemically based transfection reagents for the transfection of lymphocytic cell lines such as Jurkat, even if less efficient than electroporation techniques, especially for primary lymphocytes. Transfection of Jurkats with jetPEI™ has been previously reported (Goffin V., et.al., 2005, Nucleic Acids Res 33: 4285-4310. Soller M., et al., 2006, J Leukoc Biol 79: 235-243, Abbady A. et al., 2005. Biochem Pharmacol 70:570-579.). Optimal protocol conditions are also available here.
