How early can I start to detect gene expression in transfected protoplasts?
A14. It depends on the amount of DNA you use and the type of genes you introduce. I can detect LUC reporter gene expression as early as 30 min after DNA transfection and as early as 1-2 h for GFP expression. However, each reporter gene and response may be different and you need to spend some time to figure out what the optimal incubation time is for you assays.
