How does VayTeks MicroTome software work?
In part, MicroTome does in software what the confocal microscope does by virtue of hardware (i.e. the pinhole). Both systems use image processing but MicroTome is more flexible and can be used to great advantage with conventional microscopy and to improve confocal microscopy. MicroTome software, as illustrated in Figure 2, uses a standard white-light microscope and requires no special attachments. A video camera captures and digitizes the images from the microscope, which are then stored by a computer. Image enhancement algorithms are used to deconvolve the image, i.e. remove the blur or haze contributed by the out-of-focus image planes. The algorithms used by MicroTome have the same function as the apertures in the laser scanning confocal microscopes – removing the out-of-focus portion of the image. You can transform your standard microscope and your computer by simply adding VayTek’s deconvolution software package.
