How does Time-Resolved Picosecond Fluorescence Lifetime Imagig Microscopy work?
The fluorescence is excited by a laser pulse at appropriate wavelengths. Because the auto fluorescence typically decays with lifetimes on the scale of 1ns the excitation pulse of the laser has to be even shorter. The picture below scematically shows periodically appearing excitation pulses and the according decay curves of the auto fluorescence signal. The decay lifetime is measured with our 4 Picos high speed ICCD camera, that can be gated as short as 200ps. The vertical bars in the picture show the points in time where the camera is gated, i.e. where the shutter is opened. As can be seen, the gating windows scan the decay curve starting from the point of excitation down to minimum intensity. This scan is easily performed by increasing the time delay of the gating window with respect to the point of excitation. Our programmable built-in trigger delay unit provides these delay intervals that can be adjusted in steps of 10ps starting at zero.
Related Questions
- The Modulus Microplate detects luminescence, fluorescence, and absorbance. Can it also read fluorescence polarization (FP) and time-resolved fluorescence (TRF)?
- How does Time-Resolved Picosecond Fluorescence Lifetime Imagig Microscopy work?
- How can we calculate the lifetime of fluorescence(S1) AND phosphorescence(T1)?
