How does the Universal Test plate analysis determine the pass/fail criteria for accuracy and repeatability?
Your Universal Test Plate comes with a tri-fold paper listing absorbance values at different wavelengths for your plate. (example below) These will be referred to as the “target” values. For Accuracy measurements, the measurement on your instrument has to lie within specified limits of your target value for that wavelength as follows: (Target – error) < measurement < (Target + error) Where error = 1% + 0.01 + instrument specification The instrument specification is found in the "Specifications" section of your Operator's Manual. It depends on factors such as the read type and plate type and OD of the measurement. Example: For a Synergy HT, the accuracy specification according to the Operator's Manual in the range of 0.000-2.000 OD for a normal-mode read of a 96-well plate is + 1% + 0.010 OD The total error in this case will be + 2% + 0.020 (the sum of the errors of the instrument and the plate). The target value (from the trifold plate insert) is 0.149 for this example, so the error wo
