How does the term “gain” apply to the NanoDrop 3300?
Gain as it applies to a fluorometer classically refers to a means of controlling the excitation source voltage to increase the excitation of fluorphores. The NanoDrop NanoDrop 3300 fluorospectrometer applies a constant current for each measurement while using time as a means of maximizing the excitation of the fluorophore. NanoDrop uses the term “gain” to refer to the amount of time the sample is excited. Time is therefore used to maximize the signal to the CCD spectrometer detectors while preventing detector saturation.
