How does the TAP Express System work?
There are two PCR steps involved in the TAP Express process. The first PCR step incorporates unique TAP DNA sequences onto the 5′ and 3′ ends of the gene-of-interest via gene-specific TAP PCR primers. The second PCR step incorporates the high efficiency TAP Express Promoter and TAP Express Terminator onto the first-step PCR product. After the second PCR step is complete, the gene-of-interest is ready for expression.
