How does the staining pattern differ between CyDye™ DIGE Fluor minimal dyes, fluorescent post-labelling and silver-stained gels?
There is no difference between the 2-D spot pattern you see with CyDye DIGE Fluor minimal dye labelling, fluorescent post-labelling, and post-staining with silver. The mass increase resulting from attachment of a CyDye DIGE Fluor to a protein will shift the 2-D spot pattern to the same extent for each protein in the sample so all the labelled proteins will remain in the same place relative to one another.
