How does the protocol for staining with eFluorTM Nanocrystal conjugated antibodies differ from staining with conventional organic fluorochrome conjugated antibodies?
The eFluorTM Nanocrystals conjugated antibodies may be used in the same fashion as other fluorochromes, although we recommend the use of the eFluorTM NC Flow Cytometry Staining Buffer (cat. 00-3222) for optimal performance. eFluorTM NC Flow Cytometry Staining Buffer is fully compatible with other fluorochromes. The antibodies are provided as test sizes pre-diluted to the optimal concentrations for ease of use. Please refer to the product Technical Data Sheet.