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How does the protocol for staining with eFluorTM Nanocrystal conjugated antibodies differ from staining with conventional organic fluorochrome conjugated antibodies?

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How does the protocol for staining with eFluorTM Nanocrystal conjugated antibodies differ from staining with conventional organic fluorochrome conjugated antibodies?

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The eFluorTM Nanocrystals conjugated antibodies may be used in the same fashion as other fluorochromes, although we recommend the use of the eFluorTM NC Flow Cytometry Staining Buffer (cat. 00-3222) for optimal performance. eFluorTM NC Flow Cytometry Staining Buffer is fully compatible with other fluorochromes. The antibodies are provided as test sizes pre-diluted to the optimal concentrations for ease of use. Please refer to the product Technical Data Sheet.

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