How does the pixel size of the camera relate to the image resolution?
The pixel size of the camera and the magnification of the image determine the resolution of the image. The resolution of the image (how many microns does each pixel in the image represent) is calculated by dividing the camera pixel size by the magnification of the objective. For example, when using a the 20x objective on the BD Pathway™ bioimager, which has a camera pixel size of 6.4 µm, the resolution of the image is 6.4/20 = 0.32 µm/pixel. Note: Binning has an effect on the resolution of the image. A binning of 2×2 means that an area of four adjacent pixels has been combined into one larger square pixel that is now 6.4 x 2 = 12.8 µm in size. Now the resolution for a 20x objective is: 12.8/20 = 0.64 µm/pixel.
