How does the Pierce 660 nm Protein Assay Kit work?
The assay is based on a dye-metal complex that binds to proteins in an acidic solution. Upon binding, the reddish dye-metal complex changes to green, resulting in an absorbance shift measurable at 660 nm. The dye interacts mainly with basic residues, such as histidine, arginine and lysine and to a lesser extent, tyrosine, tryptophan and phenylalanine.
