How does the LAMP-reaction work in principle?
LAMP which stands for Loop-mediated Isothermal Amplification is a simple, rapid and specific nucleic acid amplification method solely developed by Eiken Chemical Co., Ltd. It is characterised by the use of 6 different primers specifically designed to recognize 6 or 8 distinct regions on the target sequence. The reaction utilises a DNA Polymerase with strand displacement activity and proceeds at a constant temperature of around 65°C. Amplification and detection of the target sequence can be completed in a single step.
