How does the BioPORTER Reagent work?
When the BioPORTER reagent is combined with a protein in solution, it reacts quickly and interacts non-covalently with the protein, creating a protective vehicle for immediate delivery into cells. The BioPORTER/protein complexes are added onto cells growing in culture, where the complexes can attach to cell’s negatively charged plasma membranes. The complexes then fuse directly with the plasma membrane, delivering the captured protein into the cell’s cytoplasm, or the complexes are endocytosed by the cells and then fuse with endosomes, releasing the BioPORTER-captured protein into the cytoplasm. Delivery of functional proteins or peptides with the BioPORTER reagent is simple, robust, and efficient, requiring only a 4-hour incubation for most cell types.
