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How does SPERM HY-LITER stain hidden sperm?

April 26, 2017hidden hy-liter sperm stain

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How does SPERM HY-LITER stain hidden sperm?

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The protocol has been specifically designed to allow the Alexa488 tagged monoclonal antibody to reach sperm at all layers of the stained slide. As fluorescent detection is not inhibited by cell layers, sperm under cells, over cells, and at all layers of the preparation are easily identified.