Important Notice: Our web hosting provider recently started charging us for additional visits, which was unexpected. In response, we're seeking donations. Depending on the situation, we may explore different monetization options for our Community and Expert Contributors. It's crucial to provide more returns for their expertise and offer more Expert Validated Answers or AI Validated Answers. Learn more about our hosting issue here.

How does SABiosciences control the quality of the RT² qPCR Primer Assays?

April 26, 2017assays CONTROL primer qPCR quality sabiosciences

0

Posted

How does SABiosciences control the quality of the RT² qPCR Primer Assays?

1 Answer

0

Posted

Each RT² PCR Primer Assay is validated at SABiosciences, with both a real-time and conventional PCR quality control assay. These assays are carried out using a single source of genomic DNA. In order to pass QC, each primer assay must generate a single band of the correct predicted size by agarose gel electrophoresis and a single peak in the real-time dissociation curve without the appearance of primer dimers. The amplification efficiencies (DART method) and sensitivities of each primer set are also validated.