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How does rSeq the 95% confidence intervals for gene expression estimates?

April 26, 2017confidence estimates expression gene intervals rseq

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How does rSeq the 95% confidence intervals for gene expression estimates?

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The 95% confidence intervals are computed by using normal approximation of binomial random variables, i.e., we assume the number of reads mapped to a gene is a binomial random variable with parameters (p, N), where p is the gene expression, and N is the total number of mapped reads.