How does reverse transcriptase make double stranded DNA from RNA?
After using reverse transcriptase to synthesize the first strand, you add an RNase to degrade the RNA leaving the single-stranded DNA. The single stranded DNA folds back on itself to make a hair pin loop that acts as a primer for the reverse transcriptase to synthesize the second DNA strand. Or a DNA polymerase can be used for second strand synthesis with a primer.
