How does RAST work?
• RAST applies FIG’s “Subsystem Approach” for High-Throughput Comparative Analysis to rapidly call and annotate the genes of an essentially complete genome using a “Highest Reliability First” strategy based on FIG’s collection of manually curated Subsystems and subsystem-derived Protein Families. RAST’s subsystem-based approach automatically ensures a high degree of annotation consistency, and delivers its data in a format designed to support high-throughput genome annotation projects. The RAST strategy proceeds as follows: • Find RNAs. (Currently, rRNAs and tRNAs; eventually, RFAM RNAs and other small RNAs.) • Find gene candidates for “Special Proteins.” (Currently, selenoproteins and pyrrolysoproteins; eventually, candidates for genes with programmed frameshifts, etc.) • Find gene candidates for membership in the “Universal” Protein Families (tRNA Synthetases, etc.); as a side-effect, estimate phylogenetic neighborhood of genome. • Find gene candidates for membership in the FIGfams a
Related Questions
- I selected "Keep existing gene calls" and uploaded a GenBank file, but RAST failed with the cryptic error "Zero-size or non-existent FASTA file." What does this mean?
- RAST is complaining about "Duplicate contig IDs," but all my contig IDs appear unique to me. Whats going on?
- Can RAST tests be done in place of skin testing?
