How does qPCR standard differ from PCR product cloning?
These two services differ in several aspects that are derived from their use. qPCR standards are primarily designated to be used in real-time PCR, and therefore it is assumed that the insert will be approximately 500 bp long, and the customer will obtain the plasmid in a concentration suitable to prepare the calibration curves. Plasmid selection depends on how the PCR product is prepared, and this selection cannot be influenced by the customer. As for cloning, PCR product length is limited only by technical capabilities of the cloning process (approximately 3 – 4 kb, depending on the plasmid type). The customer will obtain such a concentration of the plasmid that is suitable for various ways of use (further cloning, transfection etc.).
