How does one validate assays?
• After one designs the assays, they should be validated. Validation can help remove/fix problem assays before putting them into production. You can validate assays by running it in at least 24 wells of a plate. Two or more wells can be “No Taq controls” to show any primer-primer interactions. Two or more wells can be “No template controls” to show any DNA contamination problems and the remaining wells can be run with known good DNA (such as commercially available DNA samples). A performing assay should show good extension yield and clear clusters. If the assay looks good here, then you can proceed to production with these assays.
